FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499734
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT66941.33951262071422No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG27980.5598978656645336No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22740.45504208238783034No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22070.44163494979329004No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA17740.35498885407036546No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16680.33377756966706285No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14610.2923555331436324No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT13780.27574669724293327No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13400.2681426518908059No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13160.2633400969315676No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12860.2573369032325197No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12470.24953275142375742No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12440.24893243205385265No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12410.24833211268394786No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG12270.24553062229105885No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12150.2431293448114397No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC11990.23992764150528081No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11700.23412455426286785No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11450.2291218928469946No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11020.22051731521169263No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10910.2183161441887084No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10790.21591486670908927No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10660.21331348277283516No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10580.21171263111975572No Hit
CGTTCCTACGACGCCGATGACCGCCTGACCG10500.21011177946667625No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10270.2055093309640729No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10090.20190741474464413No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG9960.19930603080839004No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9480.18970092088991344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9440.18890049506337372No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9370.18749974986692922No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9230.1846982594740402No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG8760.17529325601219847No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8460.1692900623131506No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8390.1678893171167061No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG8010.16028527176457874No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG7770.15548271680534045No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7720.15448218452216578No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7650.15308143932572127No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7130.14267590358070492No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7100.14207558421080016No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6880.13767324216483168No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6750.13507185822857762No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6750.13507185822857762No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6750.13507185822857762No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6630.13267058074895843No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6250.12506653539683113No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6140.12286536437384689No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG6120.12246515146057703No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5830.11666206421816407No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5770.1154614254783545No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5720.11446089319517984No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5580.11165940280229082No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5530.11065887051911617No Hit
ATCTCCCAGATCCAGCGTCCGGTTAAAGAAC5410.10825759303949702No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5200.10405535745016349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)