FastQCFastQC Report
Sun 21 May 2023
EGAF00007960076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472451
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG27680.5858808638356147No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27500.582070944923389No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24920.5274621071814855No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG19120.40469805334309805No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15110.31982152646517836No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14090.29823198596256545No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG13040.2760074589745815No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12640.2675409725029686No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG12180.2578045130606137No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG11890.25166631036869436No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11820.25018467523616206No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11410.24150652660275881No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG11360.24044821579380718No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA11130.23557998607262975No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10860.22986510770429103No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10760.22774848608638779No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10510.22245693204162972No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT10480.22182194555625873No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG10300.21801202664403294No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9740.20615894558377482No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG9730.20594728342198448No Hit
GGTACGGTGAGTGGCACGGTCCTTCAGGTCGTAACGGGTGCTAG9640.2040423239658716No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9550.2021373645097587No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9490.20086739153901675No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG9160.1938825401999361No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9110.19282422939098445No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9100.19261256722919415No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8920.18880264831696833No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG8910.188590986155178No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8080.17102302672658118No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG7860.16636645916719406No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7570.16022825647527467No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7550.15980493215169403No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG7430.15726498621021018No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7330.15514836459230694No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC7070.14964514838575854No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA7020.1485868375768069No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6920.14647021595890367No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6730.14244863488488754No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6280.132923837604323No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6250.132288851118952No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5820.12318737816196812No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG5720.12107075654406488No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5710.12085909438227457No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5690.12043577005869392No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5680.12022410789690359No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5590.1183191484407907No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5460.11556754033751647No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG5400.11429756736677453No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5300.1121809457488713No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5090.10773604035127451No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5060.10710105386590356No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5010.10604274305695194No Hit
AACAGAGGACAGACGGTCACGGGTCTCACGAGAACCGCGTTCAC5010.10604274305695194No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4760.10075118901219386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)