FastQCFastQC Report
Wed 8 May 2024
EGAF00007960077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472451
Total Bases14.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62501.3228885111895203No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45760.968566052352519No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG26800.5672545935980663No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21500.45507364784919496No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG20500.4339074316701626No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15440.3268063778042591No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA15220.32214981024487194No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13290.28129901301933957No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT12750.2698692562826621No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12430.26309606710537176No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12150.2571695265752427No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11660.2467980806475169No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11510.24362314822066203No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11500.24341148605887172No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG11410.24150652660275881No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11230.23769660769053297No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11190.23684995904337172No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG11060.2340983509400975No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10730.22711349960101682No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC10720.22690183743922648No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10670.2258435266302749No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9880.20912221584883936No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9800.20742891855451678No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9740.20615894558377482No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9730.20594728342198448No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9710.20552395909840385No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9610.20340733748050063No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9320.19726913478858127No Hit
CGTTCCTACGACGCCGATGACCGCCTGACCG9300.19684581046500058No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9080.19218924290561348No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG8420.17821954022745215No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8000.1693297294322586No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7890.16700144565256503No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7870.16657812132898436No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7510.15895828350453273No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG7510.15895828350453273No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7400.15662999972483918No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG7230.1530317429744037No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7210.15260841865082306No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6750.14287195920846818No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6660.14096699975235527No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6630.1403320132669843No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6600.13969702678161333No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6190.13101887814821006No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6120.12953724301567782No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6120.12953724301567782No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG6000.12699729707419394No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5990.1267856349124036No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5870.12424568897091974No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5620.11895413492616165No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5160.1092176754838068No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5110.10815936467485517No Hit
ATCTCCCAGATCCAGCGTCCGGTTAAAGAAC5080.10752437818948421No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5060.10710105386590356No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT4960.10498443224800033No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4830.10223282414472612No Hit
AACAACAATGAAGTTAAGTTCGACCTGAACG4790.10138617549756482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)