FastQCFastQC Report
Wed 8 May 2024
EGAF00007960079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505456
Total Bases15.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64811.282208540407078No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG29010.5739371973030294No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22560.4463296508499256No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22280.4407900984457599No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16960.33553860276661074No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT14040.2777689848374537No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13910.27519704979266246No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13710.2712402266468298No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13550.2680747681301636No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG12910.25541293406349913No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12700.2512582697603748No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12660.25046690513120823No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12530.24789497008641703No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12520.24769712892912538No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12130.23998132379475165No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC11970.23681586527808554No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11940.23622234180621063No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11810.23365040676141938No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11500.22751733088537873No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11230.22217561963850463No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11010.2178231141780887No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10720.2120857206166313No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10700.21169003830204805No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10590.20951378557184006No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10170.20120445696559147No Hit
CGTTCCTACGACGCCGATGACCGCCTGACCG10060.19902820423538348No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9960.19704979266246714No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA9830.19447785761767591No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9730.19249944604475958No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG9520.18834478174163527No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9310.18419011743851096No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9180.18161818239371974No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG8780.17370453610205439No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG8780.17370453610205439No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8460.1673736190687221No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8400.1661865721249723No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8300.16420816055205595No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8130.16084486087809818No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG8110.16044917856351493No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7510.1485787091260169No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7100.1404672216770599No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7100.1404672216770599No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7020.13888449241872686No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6980.13809312778956032No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6850.13552119274476906No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6790.13433414580101927No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG6560.1297837991833117No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6410.1268161818239372No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6210.12285935867810452No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5890.11652844164477223No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5790.11455003007185591No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5720.11316514197081448No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5670.11217593618435631No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5650.11178025386977303No Hit
ATCTCCCAGATCCAGCGTCCGGTTAAAGAAC5630.11138457155518977No Hit
AACAACAATGAAGTTAAGTTCGACCTGAACG5420.10722990725206547No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5280.1044601310499826No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5180.10248171947706626No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5160.10208603716248298No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5120.10129467253331645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)