FastQCFastQC Report
Sun 21 May 2023
EGAF00007960080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477253
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27140.5686711241207494No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG26350.5521180589750091No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25600.5364031237100658No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG20140.4219983949812782No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15310.32079421187504326No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14770.309479458484284No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG12750.2671538995040366No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG12610.26422044492124724No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12580.2635918475106495No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA11870.2487150421265031No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG11710.24536252260331523No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG11690.24494345766291675No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11590.24284813296092428No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11300.2367716913251462No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11130.23320963933175903No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10960.22964758733837187No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT10640.22294254829199606No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10570.22147582100060137No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9850.20638948314625577No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG9730.20387509350386482No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9670.20261789868266938No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9520.1994749116296807No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG9310.19507472975549656No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG9290.19465566481509808No Hit
GGTACGGTGAGTGGCACGGTCCTTCAGGTCGTAACGGGTGCTAG9160.1919317427025079No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9150.19172221023230865No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG9040.18941735306011698No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8990.18836969070912074No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8690.1820837166031434No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8540.17894072955015472No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG7900.1655306514574031No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7810.1636448592256099No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7500.1571493526494333No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG7470.15652075523883557No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7380.15463496300704238No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA7320.15337776818584692No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC7070.1481394564308658No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6780.14206301479508773No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6290.13179592375532476No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6240.13074826140432852No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6140.12865293670233607No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6090.12760527435133986No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5880.12320509247715572No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5740.1202716378943663No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5680.11901444307317083No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG5510.11545239107978368No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5450.1141951962585882No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5430.11377613131818973No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5310.11126174167579879No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5270.11042361179500182No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5130.10749015721221239No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5110.1070710922718139No Hit
AACAGAGGACAGACGGTCACGGGTCTCACGAGAACCGCGTTCAC5110.1070710922718139No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG4910.102880442867829No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4810.10078511816583657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)