FastQCFastQC Report
Wed 8 May 2024
EGAF00007960081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477253
Total Bases14.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61751.2938630034803345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41620.8720741409692553No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG25780.5401747081736521No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22160.46432395396152565No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG21330.44693275893498835No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15630.327499250921419No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA13980.2929263933385437No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13040.27323034113981476No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12490.26170605527885626No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12420.2602393279874616No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12300.25772493834507065No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT12210.25583914611327746No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG11800.24724831483510842No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG11610.24326719790132276No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11450.23991467837813488No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11430.2394956134377364No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11430.2394956134377364No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11270.23614309391454846No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11080.23216197698076282No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC10950.22943805486817262No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10660.22336161323239456No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10280.21539937936482326No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10160.21288498972243233No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10000.20953247019924442No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9930.2080657429078497No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9840.2061799506760565No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9820.205760885735658No Hit
CGTTCCTACGACGCCGATGACCGCCTGACCG9020.18899828811971847No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG8940.1873220283581245No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG8920.18690296341772603No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8910.1866934309475268No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8660.18145511919254567No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7960.16678784627859855No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7880.1651115865170046No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG7660.16050187217262124No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7500.1571493526494333No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG7470.15652075523883557No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7310.1531682357156477No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7140.1496061837222605No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6780.14206301479508773No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6780.14206301479508773No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6640.1391295602122983No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6480.1357770406891104No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6430.13472937833811416No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6270.13137685881492625No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6010.1259290145897459No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5970.12509088470894894No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5900.12362415741755421No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5860.12278602753675724No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5740.1202716378943663No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5340.11189033908639652No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5330.11168080661619728No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5260.11021407932480255No Hit
ATCTCCCAGATCCAGCGTCCGGTTAAAGAAC5230.10958548191420484No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT4930.10329950780822751No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4890.10246137792743051No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4860.1018327805168328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)