FastQCFastQC Report
Wed 8 May 2024
EGAF00007960115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444921
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49911.1217721797802307No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25020.562347023404155No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15490.3481516943457378No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13060.29353525682087384No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11930.2681374895768013No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11350.25510146745152507No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10700.24049213231112937No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10330.23217604923121182No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8660.19464129587050286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8450.18992135682514422No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8400.18879756181434457No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8160.18340334576250614No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7610.17104160064370977No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7150.16070268654435282No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7030.1580055785184336No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6870.15440943448387467No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6630.14901521843203624No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6570.14766666441907664No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6490.14586859240179717No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6300.14159817136075842No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5850.1314840162635614No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5660.12721359522252265No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5530.12429172819444351No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5510.12384221019012365No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5280.11867275314044515No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5250.11799847613396534No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5240.11777371713180543No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4840.10878335704540805No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4680.10518721301084911No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4660.10473769500652926No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA4590.10316438199140972No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4520.10159106897629018No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG4490.10091679196981038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)