FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448131
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47641.0630820005757244No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA24890.5554179469842524No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16820.37533667610587085No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12960.2892011487712298No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11490.25639824069301165No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11440.25528249552028315No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10940.24412504379299804No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10670.23810001986026408No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8690.19391651102021507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8550.19079242453657524No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8550.19079242453657524No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8440.1883377851565725No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7520.16780807397836794No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7080.15798951645835704No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7060.15754321838926563No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6940.15486542997471722No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6750.15062559831834887No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6670.14884040604198326No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6450.14393112728197782No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6430.1434848292128864No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5850.1305421852092357No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5710.12741809872559587No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5630.12563290644923022No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5600.12496345934559314No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5540.12362456513831893No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5450.12161622382740761No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5310.11849213734376778No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4820.10755783465102838No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4730.10554949334011707No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4650.10376430106375145No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4510.10064021458011162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)