FastQCFastQC Report
Wed 8 May 2024
EGAF00007960130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463893
Total Bases20.4 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT29890.6443296191147527No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28580.6160903484208643No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC24430.5266300633982406No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19590.4222956586971564No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18240.393194120195821No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13900.29963806308782415No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG11250.24251282084446196No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10140.21858488918780838No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC10140.21858488918780838No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG8270.1782738691896623No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8110.17482479795987435No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8010.17266912844125692No Hit
TTTGGCAACAACCTGTACTGGCGGCACCGGCGGCGGGCTCAGGT7790.16792665550029856No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7640.1646931512223724No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7600.1638308834149254No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7390.1593039774258288No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC6490.13990295175827183No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6350.13688501443220744No Hit
ATTGGCTGCTTGGGTAGCCAGTTCGTTCATGCGCTGCAGAATGC6240.13451377796172823No Hit
ACGATCGGTGTCTTTGGAGTTAGTACCGTTCGCTGCCTGAACTG6160.13278924234683429No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC6020.12977130502076986No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5940.12804676940587592No Hit
ATCACGGTCAGACTGCGCGTTGGTACCGTTTGCGGACTGAACTG5910.1274000685502907No Hit
AGTAGAGGCACGGTCCAGACCCTTGATTTGTTTGCGCATTTTTT5810.12524439903167325No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5750.12395099732050278No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5710.12308872951305581No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5700.12287316256119407No Hit
GGTGGAAGCCTGATCCAGGCCTTTAATCTGTTTGCGCATCTTTT5600.12071749304257663No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5500.11856182352395919No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5430.117052854860927No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5370.11575945314975652No Hit
GCTAGAGGCCTGAGACAGGCCGCGGATCTGCTTACGCATTTTTT5260.11338821667927733No Hit
CAGGGAGTTAGTCAGAGAGGTCAGGCCGCTAGACGCGGTCTGCA5140.11080141325693639No Hit
GTTGTCAGACGCTTTGTTCAGGCCACGAATCTGGGAGCGCATTT4960.10692120812342501No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA4940.10649007421970154No Hit
ATCGCTGCTGTTGTTGGTAGAGTTCGCGGCCTGAACGGACAGTT4770.10282543603805187No Hit
AGAGGAACCCGCGGTAGACGGACCTGCGGTAGCTGCTTCGGAAC4680.10088533347129619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)