FastQCFastQC Report
Wed 8 May 2024
EGAF00007960131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463893
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA65581.4136880703093169No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35400.7631070095905736No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA32550.7016704283099766No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19440.4190621544192303No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT18540.3996611287516733No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC10760.23195004020323653No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10600.22850096897344863No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG10540.22720756726227814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9210.19853716266466623No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8520.18366304298620587No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA8230.1774116013822153No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8150.17568706576732135No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT8090.17439366405615087No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7940.1711601597782247No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7290.15714830790721138No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7230.1558549061960409No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7070.15240583496625298No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG6720.14486099165109195No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6590.14205862127688929No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6530.14076521956571883No Hit
ATCAACCGTGCAGCGGACGATGCGGCTGGTC6490.13990295175827183No Hit
GGCCTGACCATTAGCGAGAAGATGCGTAAAC6480.1396873848064101No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6410.1381784161433779No Hit
GGTCTGACCATTAGCGAAAAAATGCGCAAAC6380.13753171528779265No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6170.13300480929869601No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA6010.12955573806890813No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5940.12804676940587592No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA5850.12610666683912022No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA5700.12287316256119407No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5680.12244202865747057No Hit
GCGGCTGGTCTGGCAATTTCTCAGCGCATGA5630.12136419389816187No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5480.1181306896202357No Hit
AAACGTATCAACAGCGCTGCCGACGACGCAG5350.11532831924603304No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5170.11144811411252165No Hit
CGCTGCACCGTAAGCGGTGTCGAATATTCTC5120.11037027935321292No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG5050.10886131069018071No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4830.10411883774922234No Hit
ATGACCACTAAACTGATCAACCTGCCACACC4730.10196316823060492No Hit
GAACTGACCAGCGGCGGCGGTGAAACCCCGA4710.1015320343268814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)