FastQCFastQC Report
Wed 31 May 2023
EGAF00007960134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469662
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT30100.6408864247054264No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28810.6134198636466225No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC25210.5367689955755416No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19550.41625679744156435No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18460.3930486179422649No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14720.31341688277953084No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG11350.24166315350188008No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC11010.23442390485072245No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10340.2201583266263824No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA8360.17800034918728788No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8120.1728902913158825No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG8000.1703352623801798No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG7930.16884482883435323No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC7800.1660768808206753No Hit
TTTGGCAACAACCTGTACTGGCGGCACCGGCGGCGGGCTCAGGT7620.16224433741712124No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6790.14457205394517758No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC6650.14159118685352445No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC6610.14073951054162356No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG6110.13009355664286232No Hit
ATTGGCTGCTTGGGTAGCCAGTTCGTTCATGCGCTGCAGAATGC6040.12860312309703575No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6010.12796436586311005No Hit
GCTAGAGGCCTGAGACAGGCCGCGGATCTGCTTACGCATTTTTT5930.12626101323930827No Hit
AGTAGAGGCACGGTCCAGACCCTTGATTTGTTTGCGCATTTTTT5860.1247705796934817No Hit
GGTGGAAGCCTGATCCAGGCCTTTAATCTGTTTGCGCATCTTTT5830.12413182245955602No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5760.12264138891372944No Hit
ACGATCGGTGTCTTTGGAGTTAGTACCGTTCGCTGCCTGAACTG5740.12221555075777901No Hit
ATCACGGTCAGACTGCGCGTTGGTACCGTTTGCGGACTGAACTG5740.12221555075777901No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5670.12072511721195242No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5580.1188088455101754No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5550.11817008827624974No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5400.11497630210662135No Hit
CAGGGAGTTAGTCAGAGAGGTCAGGCCGCTAGACGCGGTCTGCA5130.1092274870012903No Hit
GTTGTCAGACGCTTTGTTCAGGCCACGAATCTGGGAGCGCATTT5080.10816289161141417No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA5070.10794997253343894No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4910.10454326728583535No Hit
ATCGCTGCTGTTGTTGGTAGAGTTCGCGGCCTGAACGGACAGTT4780.10177531927215742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)