FastQCFastQC Report
Wed 8 May 2024
EGAF00007960135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469662
Total Bases14.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA66541.4167635448471454No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA36430.7756642010637438No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA32750.697309980368861No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19600.4173213928314405No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT18550.39496488964404186No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG11400.24272774889175622No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10590.225481303575763No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC10410.22164876017220894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8900.18949797939795002No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8460.18012953996704012No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA8410.179064944577164No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8330.1773615919533622No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT8070.17182569592600636No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7950.16927066699030366No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6950.1479787591927812No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6880.14648832564695463No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG6770.14414621578922715No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6740.14350745855530148No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6590.1403136723856731No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA6390.1360552908261686No Hit
GGCCTGACCATTAGCGAGAAGATGCGTAAAC6310.13435193820236682No Hit
ATCAACCGTGCAGCGGACGATGCGGCTGGTC6300.13413901912439158No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6280.13371318096844115No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6170.13137107111071367No Hit
GGTCTGACCATTAGCGAAAAAATGCGCAAAC6140.13073231387678796No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6080.12945479940893664No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA6040.12860312309703575No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6030.1283902040190605No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5870.12498349877145692No Hit
GCGGCTGGTCTGGCAATTTCTCAGCGCATGA5860.1247705796934817No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA5860.1247705796934817No Hit
AAACGTATCAACAGCGCTGCCGACGACGCAG5300.11284711132686912No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG5240.11156959685901775No Hit
CGCTGCACCGTAAGCGGTGTCGAATATTCTC5210.11093083962509208No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5200.11071792054711685No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5100.10858872976736461No Hit
ATGACCACTAAACTGATCAACCTGCCACACC5060.10773705345546371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)