FastQCFastQC Report
Wed 8 May 2024
EGAF00007960141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276859
Total Bases8.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28361.0243481338876468No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15900.5742995532021715No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15510.5602129603877787No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA14720.5316785800714443No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10570.38178278473880206No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA8790.3174901303551627No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7160.25861539628475144No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6580.2376661044069364No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG6160.22249592752989789No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5820.21021530815324768No Hit
AGCACCCCGCGTGCGGCTGTCACCCAGACGG5290.1910719897131753No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5190.18746004283768994No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5180.1870988481501414No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5150.1860152640874958No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5040.18204212252446192No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5040.18204212252446192No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4850.17517942346103973No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4820.17409583939839413No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4510.16289880408438953No Hit
GCGCTGGCTCGCCTGGACGCCGAACAGCGTG4410.15928685720890415No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4000.14447787501941423No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG3970.14339429095676862No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC3950.14267190158167153No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA3660.13219725564276402No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG3430.12388977782914769No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT3260.11774946814082259No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG3240.11702707876572552No Hit
GATCCACTGTTCGACCAGGCGGTCCAGTTCG3230.11666588407817699No Hit
CCGAAAGTGTCCGACACCCCGAAAGCCCCGG3210.11594349470307991No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG2940.10619123813926945No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC2880.10402407001397823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)