FastQCFastQC Report
Wed 8 May 2024
EGAF00007960151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420225
Total Bases13 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17960.42739008864298883No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15680.37313344041882324No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13430.3195906954607651No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13410.31911475995002675No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12660.30126717829734073No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12150.2891308227735142No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT12030.28627520970908443No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10710.2548634660003569No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10200.24272711047653045No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9210.21916830269498483No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8170.19441965613659348No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7770.1849009459218276No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7110.16919507406746387No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6880.16372181569397345No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC6620.15753465405437564No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6530.1553929442560533No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6390.15206139568088525No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC6380.1518234279255161No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6370.15158546017014696No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5910.14063894342316616No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5790.1377833303587364No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5760.13706942709262895No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5700.13564162056041407No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5660.13468974953893748No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5570.13254803974061513No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5530.13159616871913857No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5420.12897852341007796No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5210.12398120054732584No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5160.12279136177048008No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5020.11945981319531204No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4980.11850794217383544No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT4950.11779403890772801No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4750.11303468380034504No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA4750.11303468380034504No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4700.11184484502349931No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4570.1087512642037004No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4530.1077993931822238No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4510.1073234576714855No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4310.10256410256410256No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4300.10232613480873343No Hit
GACGGTACCTCTAACGCCCTGTACCGTACCA4260.10137426378725684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)