FastQCFastQC Report
Wed 8 May 2024
EGAF00007960157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403443
Total Bases12.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38030.9426362584057723No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT35380.8769516387692933No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18370.45533074064985635No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16690.41368917046522063No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG15820.39212478590531996No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC13990.3467652183827703No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG12770.31652550670107055No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT11630.2882687269329249No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11330.28083273225709704No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC9800.2429091594103752No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC8840.21911397644772618No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7560.18738706583086087No Hit
GACGTCGGTGGCGAAGGTGCAGAAAAAATGT7290.18069467062261582No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC7250.1797032046658388No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7110.17623307381711914No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6850.16978854509806837No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5790.14351469724347676No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG5550.13756590150281453No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5440.13483937012167765No Hit
ACCGACGTTCTGGAATCCGCGACTGCAGACA5330.13211283874054078No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5190.12864270789182114No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4930.12219817917277039No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT4750.11773658236727369No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4620.1145143180077483No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA4530.11228351960499995No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA4530.11228351960499995No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4400.10906125524547457No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA4280.10608685737514345No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4130.10236886003722956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)