FastQCFastQC Report
Wed 8 May 2024
EGAF00007960158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433378
Total Bases19 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT38570.8899851861423514No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT19470.44926138382659014No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG16220.3742691137990392No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG14520.335042387938474No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG13100.3022765345725902No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT12490.28820106235203446No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12290.2835861534272621No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT9650.22266935562026682No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8940.2062864289373249No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT7790.1797507026198838No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7780.17951995717364516No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT7680.177212502711259No Hit
CCACTGCGCGATGCCGAAGGCACCGGAGGAAGCATTCTTCGCGT7280.16798268486171428No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG7100.16382926682941912No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG6100.1407547222055573No Hit
CCAGAAGGTACGGATCTGGTTTTTGCGTTTATCGTTCAGGGCAC5910.13637055872702353No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC5760.13290937703344424No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG5550.12806372266243327No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5390.12437179552261537No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC5340.12321806829142226No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5300.12229508650646778No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT4920.1135267595494003No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA4870.1123730323182072No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT4750.10960408696334377No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4660.1075273779471962No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA4570.10545066893104865No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG4340.10014352366756042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)