FastQCFastQC Report
Wed 31 May 2023
EGAF00007960171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417339
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46461.1132436700140653No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40660.974267921282219No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA32410.7765868993791618No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30980.7423221889159652No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23880.5721967033993947No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG23790.5700401831604524No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18640.4466393028209681No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18330.43921128866461084No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15650.3749949082161025No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15530.37211954789751256No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14510.3476789851894982No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14070.3371359973546685No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13920.3335417969564311No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11670.27962879098287No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11220.26884618978815783No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9480.22715346516860394No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8850.21205782349600685No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7980.1912114611862299No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7940.19025300774669993No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7850.18809648750775748No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7850.18809648750775748No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6970.16701051183809804No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG6940.16629167175845055No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6820.16341631143986063No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6650.15934288432185825No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6240.14951873656667602No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6020.14424724264926117No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5720.13705884185278636No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5510.132026961295254No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5420.12987044105631154No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5410.12963082769642903No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5300.1269950807377216No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4970.11908783986159933No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC4970.11908783986159933No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4830.11573325282324441No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4620.11070137226571206No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4610.11046175890582954No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC4530.1085448520267696No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4530.1085448520267696No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC4310.10327335810935476No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA4260.10207529130994226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)