FastQCFastQC Report
Wed 8 May 2024
EGAF00007960174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420880
Total Bases18.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24240.5759361338148641No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG23150.5500380155863904No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22040.5236647025280365No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21390.5082208705569283No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19060.4528606728758791No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17190.4084299562820757No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15850.37659190268009884No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15750.37421592853069763No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15500.36827599315719445No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13120.3117278084014446No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13040.3098270290819236No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12890.3062630678578217No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11310.2687226762972819No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11150.2649211176582399No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10990.26111955901919787No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9050.21502566052081354No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8470.20124501045428625No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8170.1941170880060825No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT8170.1941170880060825No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT7470.1774852689602737No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7380.17534689222581257No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7380.17534689222581257No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT7240.17202052841665083No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7120.16916935943736933No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7050.16750617753278843No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6480.15396312488120129No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT6190.14707279984793764No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6020.14303364379395553No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5670.13471773427105113No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG5500.130678578217069No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5400.12830260406766775No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5340.126877019578027No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5140.12212507127922448No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT4990.1185611100551226No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4820.11452195400114046No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4770.11333396692643984No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG4690.11143318760691884No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT4660.11072039536209846No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4480.1064436418931762No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4240.1007413039346132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)