FastQCFastQC Report
Wed 8 May 2024
EGAF00007960239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420169
Total Bases13 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47541.1314494881821362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35790.851800109003758No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT17720.4217350637481585No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA15050.3581892048199653No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG14340.341291242333442No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10640.25323143782620805No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG9970.23728547322624943No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9810.23347748167999066No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7720.18373559210698553No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7570.18016560003236792No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC7220.17183561852492688No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6770.1611256423010741No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6480.1542236576234801No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6160.14660767453096255No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5820.13851569249516266No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5490.13066170993100396No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5290.1259017204981805No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC5020.11947573476386883No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG4550.10828975959673369No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA4540.10805176012509252No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4540.10805176012509252No Hit
GCAGTCAACGAACTGACCTTCCCGGGTTCTG4540.10805176012509252No Hit

[OK]Adapter Content

Adapter graph