FastQCFastQC Report
Wed 8 May 2024
EGAF00007960293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530949
Total Bases16.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37950.7147579146019674No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA34210.6443180041774257No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG30920.582353484044607No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23490.44241537322793717No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21980.41397573024904466No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21900.41246899419718275No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17810.33543711354574546No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16490.31057596869002485No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15780.2972036862297509No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14380.2708358053221684No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13680.2576518648683772No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG13380.25200160467389526No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12490.2352391660969321No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11960.2252570397533473No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11840.22299693567555454No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG11550.21753501748755533No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10370.19531066072259295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9750.18363345632066355No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9580.18043164221045713No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9570.1802433002039744No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8520.16046738952328754No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7650.14408163495928988No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7240.13635961269349786No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6970.13127437851846413No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6930.1305210104925332No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6710.12637748634991308No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6440.12129225217487932No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6350.11959717411653473No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6280.11827878007115561No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6130.11545364997391463No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6110.11507696596094917No Hit
GCGGCTGGTCTGGCAATTTCTCAGCGCATGA5900.11112178382481179No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA5420.10208136751364066No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5390.10151634149419247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)