FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007960294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508676
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT28480.5598848776038186No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC27930.5490724940826774No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19660.38649356368297305No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18800.3695869276317342No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18000.35385982432825613No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15640.30746486958299585No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14790.29075482232305044No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14230.2797458500106158No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14190.2789594948454419No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12650.24868482098624667No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC11410.2243078108658557No Hit
GGCAATGCCGGCGCGCCAACGACCATCACGCGCTTTGTCAGTGA11040.21703402558799706No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT10920.2146749600924754No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10370.20386257657133422No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9970.19599902491959517No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9950.19560584733700823No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9410.18499005260716056No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9330.18341734227681275No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8200.16120280886065No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7760.1525529020437371No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7020.13800533148801988No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6830.13427014445344385No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6700.13171449016662867No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6660.13092813500145475No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6350.12483388247135702No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6230.12247481697583532No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6220.12227822818454183No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5790.11382491015892238No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5660.1112692558721072No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5620.11048290070693328No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5420.10655112488106377No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5140.10104663872484647No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5130.10085004993355298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)