FastQCFastQC Report
Wed 8 May 2024
EGAF00007960300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571770
Total Bases25.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT30910.5406019903107893No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT30290.5297584693145846No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26680.4666211938366826No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC23100.40400860485859696No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT22950.4013851723595152No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA20000.3497909998775731No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT18940.3312520768840618No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18120.31691064588908124No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT17570.30729139339244804No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15520.27143781590499677No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG14770.25832065340958776No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13890.24292984941497459No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT13890.24292984941497459No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13210.23103695541913707No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12960.2266645679206674No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG12950.22648967242072862No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG12460.21791979292372807No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG10810.1890620354338283No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG10430.18241600643615438No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA10110.17681935043811323No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10010.17507039543872535No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG9640.16859926194099026No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9410.16457666544239816No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9270.16212812844325517No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7640.13362016195323295No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT7630.13344526645329416No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7440.13012225195445723No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7390.12924777445476326No Hit
TGGCAGGTCCGGCTGCGGCGGCAGCATTGGACGCGCCGGGGCGG7180.12557496895604875No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6880.12032810395788515No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6490.11350717946027249No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6430.11245780646063977No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA6100.10668625496265982No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5990.10476240446333315No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5860.10248876296412894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)