FastQCFastQC Report
Wed 8 May 2024
EGAF00007960303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550880
Total Bases17 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42880.7783909381353471No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33800.6135637525413884No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29240.5307871042695324No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA28670.5204400232355504No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC21640.3928260238164391No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20060.3641446413011908No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20020.36341853035143773No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19250.3494408945686901No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT18060.3278390938135347No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA17870.3243900668022074No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17280.31367993029334884No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16790.30478507115887304No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT16450.2986131280859715No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA14400.26139994191112403No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC13520.2454255010165553No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13160.23889050246877724No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA12830.23290008713331398No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12510.227091199535289No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12110.21983009003775777No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC10880.19750217833284928No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT10860.1971391228579727No Hit
ATGTCCAACATGGACATCGATGGTATTAACA10460.18987801336044147No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10280.1866105140865524No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9640.17499273889050246No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9500.17245135056636654No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9240.16773162939297126No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8820.16010746442056348No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8170.1483081614870752No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT7810.14177316293929712No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7430.13487510891664248No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6880.12489108335753704No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6550.11890066802207376No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6320.11472553006099333No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6120.11109497531222771No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5920.10746442056346209No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5900.10710136508858555No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG5880.10673830961370898No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5820.1056491431890793No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5820.1056491431890793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)