FastQCFastQC Report
Wed 8 May 2024
EGAF00007960352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414517
Total Bases18.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27640.6668001553615412No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT26870.6482243189061003No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25780.6219286543133333No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24180.5833295136267028No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22910.55269144570669No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16320.39371123500363076No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15900.38357896057339025No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15700.37875406798756145No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13790.3326763437928963No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13380.32278531399194726No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12750.30758690234658653No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11870.28635737496893976No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11590.27960252534877944No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11270.2718826972114533No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10740.25909673185900695No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC9800.23641973670561156No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9340.22532248375820535No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8280.19975055305331266No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8230.19854432990685544No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8200.1978205960189811No Hit
TGGCAGGTCCGGCTGCGGCGGCAGCATTGGACGCGCCGGGGCGG8120.19589063898464962No Hit
TTCGTGCAGAGGATATGCCATGCCCTGGGCCGCCAGATGGATGG8040.19396068195031807No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7930.19130699102811222No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7700.1857583645544091No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7130.172007420684797No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6850.16525257106463667No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6850.16525257106463667No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6710.1618751462545565No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6620.15970394459093354No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6470.15608527515156195No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5990.14450553294557278No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5870.1416105973940755No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5840.1408868635062012No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5550.13389076925674942No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5540.13364952462745797No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5330.12858338741233774No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5210.12568845186084046No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5040.12158729316288595No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4600.11097252947406258No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4550.1097663063276054No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA4460.10759510466398242No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA4240.10228772281957073No Hit

[OK]Adapter Content

Adapter graph