FastQCFastQC Report
Wed 8 May 2024
EGAF00007960353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414517
Total Bases12.8 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55191.3314291090594594No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29570.7133603688147893No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA28500.6875471934806051No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27410.6612515288878381No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26710.6443644048374373No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17920.4323103756902612No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17210.4151820070105689No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16270.3925050118571735No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15560.37537664317748126No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14950.3606607207907034No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14070.33943119341305666No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13330.32157909084549No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12470.3008320527264262No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11670.28153248238311096No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11640.2808087484952366No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10300.24848196817018361No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10110.24389832021364627No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9960.24027965077427463No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9110.21977385728450222No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT8910.2149489646986734No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8840.2132602522936333No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8440.20361046712197567No Hit
GCGCGCGGCTCCACCTACGGTACCCCGCGTC8140.19637312824323247No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8020.1934781926917352No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7790.18792956621803208No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7520.18141596122716316No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7130.172007420684797No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6900.16645879421109386No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6380.15391407348793898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6340.1529490949707732No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6260.1510191379364417No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6080.14667673460919578No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6080.14667673460919578No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5980.14426428831628135No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5700.13750943869612103No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4910.11845111298209723No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4600.11097252947406258No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4560.11000755095689682No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4440.10711261540539954No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4420.10663012614681665No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4400.10614763688823377No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4280.10325270133673649No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4210.10156398893169641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)