FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007960357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414586
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52741.2721124205834256No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29180.7038346688021303No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27870.6722368820944268No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27710.6683776104354706No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27040.6522169103635916No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17700.42693192727202556No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17470.4213842242622761No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16190.3905100509906268No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15650.37748500914164973No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15060.3632539448992489No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14110.34033951942419666No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13160.3174250939491444No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12890.3109125730246559No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12510.301746802834635No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11970.288721760985658No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10630.2564003608419001No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10030.2419280921208145No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT9900.2387924338979126No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9380.2262498010063051No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9380.2262498010063051No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8550.20622982927547No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8540.20598862479678523No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8330.20092333074440524No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7780.18765708441674345No Hit
GCGCGCGGCTCCACCTACGGTACCCCGCGTC7590.18307419932173302No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7440.1794561321414616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7290.1758380649611902No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7250.17487324704645116No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6940.1673959082072236No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6440.15533568427298558No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6270.15123520813534466No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6100.14713473199770372No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5910.1425518469026933No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5900.14231064242400854No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5580.1345920991060962No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4820.11626055872605441No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4800.1157781497686849No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4650.1121600825884135No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4610.11119526467367447No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4510.10878321988682686No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4410.10637117509997925No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4370.1054063571852402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)