FastQCFastQC Report
Wed 8 May 2024
EGAF00007960359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403161
Total Bases12.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58141.442103774918705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31980.7932314881647778No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28050.6957518212327085No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26900.6672272367614923No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26440.6558174029730058No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25060.6215879016075464No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17380.4310932853128155No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16650.41298637517021736No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16090.39909614273205996No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14820.3675950798812385No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14510.3599058440672585No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13220.3279087014865029No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13140.32592438256676615No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13060.3239400636470294No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10930.27110757240903754No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10850.2691232534893008No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10460.259449698755584No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9210.22844471563469682No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9120.22621235684999294No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT8840.21926724063091418No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8640.21430644333157223No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA7960.19743973251380964No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7900.19595149332400702No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7470.18528577913042185No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7390.18330146021068508No Hit
GCGCGCGGCTCCACCTACGGTACCCCGCGTC7380.18305342034571798No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7380.18305342034571798No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6590.16345827101331725No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6380.15824943384900822No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6240.15477687573946886No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6160.15279255681973206No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6040.1498160784401269No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5980.14832783925032433No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5590.1386542845166075No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4970.12327581288864746No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4470.11087381964029258No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4290.10640910207088483No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4250.10541694261101645No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4220.10467282301611515No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4190.10392870342121387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)