FastQCFastQC Report
Wed 8 May 2024
EGAF00007960377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535657
Total Bases16.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64301.2003950289084246No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG38500.7187435243075326No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC34750.648736038173682No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG30700.5731279531491235No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG30570.57070102696315No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG30290.5654738013318225No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG29790.556139469847309No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC29380.548485318030008No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC28930.5400844196939459No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG26660.49770655475425507No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24050.448981344405095No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG23950.4471144781081924No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23130.4318061744735904No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC20200.3771069919743418No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC18870.35227767022553613No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17390.32464804903137645No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT17130.31979419665942943No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG16800.3136335378796506No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG15900.2968317412075264No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG15530.28992433590898653No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG15030.2805900044244731No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG14820.2766695852009775No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG14780.2759228386822164No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13050.24362605174580002No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG12830.23951894589261413No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12400.23149142081593257No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12060.22514407540646345No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG11700.21842335673761382No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11570.21599643055164033No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11450.2137561909953571No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC10610.19807451410137458No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC10510.1962076478044719No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8990.16783128009155113No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC8080.15084279678973672No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7990.1491626171225243No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7460.13926822574894007No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7260.13553449315513472No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7250.13534780652544445No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7120.13292088033947097No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG7070.13198744719101962No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6770.12638684830031158No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC6460.12059956277991327No Hit
GGTCCGGCCCACAGCAAAGAAGTTCCGGCAC5640.10529125914531126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)