FastQCFastQC Report
Wed 8 May 2024
EGAF00007960379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513463
Total Bases15.9 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64091.2481912036505065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42020.8183647117708579No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG36230.7056009878024317No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC32750.6378258998214087No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG28280.5507699678457845No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG28230.5497961878460571No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG28210.5494066758461661No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27180.5293468078517829No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC26730.5205827878542368No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG26150.5092869398573997No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG24140.47014098386836056No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG22670.4415118518763767No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG22170.4317740518791032No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19630.3823060278929543No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC19250.3749052998950265No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC17830.34724994790277003No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16470.32076313191018635No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT15840.3084935039136218No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG15280.2975871679166756No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG15110.2942763159176026No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG14650.2853175399201111No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG14630.28492802792022015No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG13920.2711003519240919No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG13500.2629205999263822No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12010.23390195593450744No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG11430.22260610793767027No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG11260.21929525593859736No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11160.21734769593914263No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10880.21189452794066954No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10810.21053123594105125No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10730.2089731879414875No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC9940.1935874639457955No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC9460.18423917594841305No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8060.15697333595604748No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC7620.14840407195844688No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7200.1402243199607372No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG6970.13574493196199142No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6820.1328235919628094No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6630.1291232279638455No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6560.12775993596422722No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6470.126007131964718No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC6270.12211201196580865No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5320.10361019197098915No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5160.10049409597186167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)