FastQCFastQC Report
Wed 8 May 2024
EGAF00007960381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534185
Total Bases16.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64331.2042644402220204No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG37510.7021911884459503No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC34910.6535189119874201No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG30550.5718992483877308No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG29390.5501839250446943No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28750.5382030569933637No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG28650.5363310463603433No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG28290.5295918080814699No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC28250.5288430038282618No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG25230.4723082827110458No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23870.44684893810196846No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG23550.4408585040763031No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21880.40959592650486254No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC19950.3734661212875689No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC18310.34276514690603443No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT17630.33003547460149574No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17610.3296610724748916No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG16540.3096305587015734No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG15550.2910976534346715No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG15410.28847683854844297No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG15000.2808015949530593No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG14790.2768703726237165No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG14250.26676151520540636No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13650.255529451407284No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG12150.22744929191197807No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12000.22464127596244746No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11950.22370527064593726No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG11880.222394863202823No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11790.22071005363310464No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11320.2119116036579088No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC10760.20142834411299454No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC10160.19019628031487218No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9030.1690425601617417No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8750.16380093038928462No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC8040.1505096548948398No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7540.14114960172973784No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7210.13497196664077052No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7110.13309995600775013No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG7060.13216395069123993No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6960.13029194005821954No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC6670.12486310922246038No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6600.1235527017793461No Hit
GGTCCGGCCCACAGCAAAGAAGTTCCGGCAC5450.10202457949961155No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5380.10071417205649727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)