FastQCFastQC Report
Wed 8 May 2024
EGAF00007960383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521319
Total Bases16.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT69421.3316222888480949No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40150.7701618394879143No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG36130.6930497449738068No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC34370.6592892259825558No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG29790.5714351481530502No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG29350.5629950184052375No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG28420.5451556532564514No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC27710.5315363529815718No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27380.5252062556707121No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG27190.5215616541887022No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG24530.47053723344056136No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG22860.43850310462499925No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG22370.42910386922402594No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21730.41682731686357105No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC19990.38345044013358426No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC17170.32935688129532975No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT16640.319190361371828No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16090.3086401991870621No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG16090.3086401991870621No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG15780.3026937441374667No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG15070.289074443862587No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG14900.2858134846418412No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG14520.27852428167782106No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG13390.2568484939163928No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12980.2489838275604764No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG12350.23689909633065356No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG12060.23133628354232247No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11550.22155340588008496No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11280.216374235353018No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11100.2129214550016401No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10990.21081142256468688No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC10300.19757576455107143No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC10230.19623301663664666No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8060.1546078312894792No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC7620.14616770154166642No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG6980.1338911491812115No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6750.12947926317667302No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6720.1289037997847767No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6600.1266019462171914No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6580.12621830395592717No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6520.12506737717213454No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC6300.12084731229822815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)