FastQCFastQC Report
Wed 8 May 2024
EGAF00007960397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488243
Total Bases15.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT81841.676214507939694No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC24580.5034378373064232No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24440.500570412683848No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23970.49094405859377405No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG22410.45899275565650705No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15470.3168504207945634No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10750.22017724780488404No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10000.20481604446965956No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9750.19969564335791812No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9640.1974426668687518No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8990.18412962397822394No Hit
GGTGCCCCGCGTGGCTCTCCAGCAACCCCGA8690.17798514264413418No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8680.17778032659966453No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8350.17102139713216574No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8320.17040694899875675No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8080.16549136393148495No Hit
CGTAAAGAACTGGAAGAAAGCGATAAATATC8070.16528654788701527No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7060.14460012739557965No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7000.14337123112876168No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6900.1413230706840651No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6480.13272079681633941No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6130.1255522352599013No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6000.12288962668179575No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5870.12022701810369017No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5770.11817885765899358No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5460.11182956028043411No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG5300.10855250356891957No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5200.10650434312422298No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5170.105889894990814No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5050.1034321024571781No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4900.10035986179013319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)