FastQCFastQC Report
Sun 21 May 2023
EGAF00007960401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488105
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA25480.5220188279161246No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21570.44191311295725305No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG21210.43453765071040046No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21060.4314645414408785No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG20350.4169184908984747No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16060.3290275657901476No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15490.3173497505659643No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG12760.2614191618606652No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12650.25916554839634914No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12640.25896067444504767No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC12530.2567070609807316No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG10950.22433697667510064No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10720.219624875795167No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA10520.21552739676913782No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10270.21040554798660124No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10180.20856168242488807No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9640.19749848905460918No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC9340.1913522705155653No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG9190.18827916124604338No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8730.17885495948617613No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8470.1735282367523381No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8470.1735282367523381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7820.16021142991774312No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6860.14054353059280278No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6860.14054353059280278No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6840.14013378269019985No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6310.12927546327122239No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6060.12415361448868582No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6040.12374386658608291No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5980.12251462287827414No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5800.11882689175484783No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5780.1184171438522449No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5620.11513916063142153No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5560.11390991692361274No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5450.11165630345929667No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5380.11022218580018645No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5280.10817344628717182No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5040.10325647145593674No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5010.10264184960203235No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4970.1018223537968265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)