FastQCFastQC Report
Wed 8 May 2024
EGAF00007960405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488935
Total Bases15.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA25360.5186783519281704No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21390.43748146481638667No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21370.4370724124883676No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20260.41437000828330967No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG19980.40864327569104275No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16100.32928712405534477No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15050.307811876834344No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG13000.2658840132123902No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12760.2609753852761615No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12390.2534079172078088No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11750.24031824271119884No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC11590.23704582408704636No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10770.2202746786382648No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG10530.21536605070203604No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA10280.2102528966017978No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10160.20779858263368342No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC10150.20759405646967385No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9790.20023111456533077No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG9680.19798132676122593No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8910.18223281213249204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8750.17896039350833956No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8160.16689334983177723No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7550.15441725382719584No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7430.15196293985908146No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7200.14725883808686227No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6610.13519179441029994No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6220.12721527401392824No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6120.1251700123738329No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6070.12414738155378527No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5840.11944327978156606No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5700.11657991348543262No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5620.11494370417335638No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5580.11412559951731824No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5430.11105770705717528No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5420.11085318089316576No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5390.11023960240113717No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5380.11003507623712763No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5350.10942149774509904No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5250.10737623610500373No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5110.1045128698088703No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5000.10226308200476546No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4930.10083139885669874No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4910.10042234652867968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)