FastQCFastQC Report
Wed 8 May 2024
EGAF00007960408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551341
Total Bases24.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC23090.41879707839612873No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22880.41498818335657967No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG22670.41117928831703066No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG18810.3411681699710343No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18090.32810910126400905No Hit
GTAGCACTCGCGTTCGAAGTCTTCGATATCAGAGTCACAGAAGG18090.32810910126400905No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17050.30924600202052815No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16730.3034419714840725No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG16330.2961869333135029No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG16220.29419179781659627No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16190.29364766995380354No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT14880.2698874199451882No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14880.2698874199451882No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14260.25864211078080535No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13280.24086726726290988No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC12650.2294405821442628No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11000.1995135496906633No Hit
TTCGCTCTGCAGCGCGTCGGTCTGGTCGGCCAGTGCTTCCAGTG10200.18500347334952416No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10150.18409659357820296No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9610.17430229204793404No Hit
CGGGCTCGGTGGGTGACGATGGTGGGTGGAAACGCCACGACGAC8790.15942946379826642No Hit
GTGCTGAACATCAAAGTCCTGCAGAACGAATTTCAGGTACTCCA8620.15634607257577435No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8610.1561646966215101No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8530.15471368898739618No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8150.14782140272535507No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7080.12841417561908147No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT7020.12732591989349604No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA6990.1267817920307033No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6730.12206601721983311No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA6650.12061500958571918No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA6320.11462960309499928No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG6290.11408547523220657No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6110.11082070805545025No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5860.10628630919884427No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5800.10519805347325883No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5790.1050166775189946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)