FastQCFastQC Report
Wed 8 May 2024
EGAF00007960409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551341
Total Bases17 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA51130.927375254153056No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36380.6598457216133028No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22910.4155323112193724No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22610.4100910325914452No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22510.40827727304880285No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG22200.4026546184666114No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG18490.3353641394345786No Hit
GGCGGCGATGACAACTCTAGCAGCGGCGGTA18040.32720222149268785No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17090.3099715058375851No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16440.29818206881040954No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT16220.29419179781659627No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG16100.2920152863654254No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15960.289476023005726No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14340.2600931184149193No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG13150.23850937985747475No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10800.19588603060537854No Hit
CCGAACGGTGGTACCCTGACTAACCTGCTGG10570.191714383657301No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA10530.19098887984024404No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10390.1884496164805447No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10370.18808686457201623No Hit
GAAGGCGACGAATTTTCCTTCTGCGATTCCG9130.16559624624325053No Hit
GCGGACGAAGAGGAAGAGGAACTGCTGGCGC8740.15852258402694522No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8690.15761570425562402No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8510.1543509370788677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8380.1519930496734326No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8230.149272410359469No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7710.13984086073772856No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7660.13893398096640738No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT7430.13476233401832985No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7140.1295024313446669No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG7140.1295024313446669No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6760.12261014508262581No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG6750.12242876912836158No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6640.12043363363145494No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG6520.11825712218028407No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6460.11716886645469864No Hit
GCTGGTCACCTGATCGGTAAAGGCGCGAAAG6340.11499235500352777No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6160.11172758782677145No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6100.11063933210118601No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5710.10356566988488068No Hit
TCTAGCGATCTGAACGCATACGGTGTAGTTT5710.10356566988488068No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5610.10175191034223828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)