FastQCFastQC Report
Wed 8 May 2024
EGAF00007960411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527549
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA46000.8719569177460293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42680.8090243749869681No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35600.6748188319947531No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23160.4390113524999574No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21700.4113361981541051No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG21040.39882551194296645No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20660.3916223895789775No Hit
GGCGGCGATGACAACTCTAGCAGCGGCGGTA18480.35029921391188307No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16220.3074595914313173No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16110.3053744770627942No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG15420.29229512329660373No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15330.2905891206314484No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT15070.2856606684876666No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14600.2767515434585223No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13290.2519197268879289No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG11750.2227281257286053No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10080.19107229849739077No Hit
CCGAACGGTGGTACCCTGACTAACCTGCTGG9730.18443784368845356No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9710.18405873198508574No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9700.18386917613340184No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9180.17401227184583803No Hit
GAAGGCGACGAATTTTCCTTCTGCGATTCCG8710.1651031468166938No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8270.15676268934260135No Hit
GCGGACGAAGAGGAAGAGGAACTGCTGGCGC8170.15486713082576217No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7670.1453893382415662No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7620.1444415589831466No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7210.13666976906410588No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6890.13060398181022045No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6680.12662330892485818No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6600.12510686211138683No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT6540.12396952700128329No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6510.12340085944623153No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG6340.12017840996760491No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG6340.12017840996760491No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6180.11714551634066218No Hit
GCTGGTCACCTGATCGGTAAAGGCGCGAAAG5680.10766772375646622No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5590.10596172109131095No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5500.10425571842615566No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5500.10425571842615566No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5420.10273927161268431No Hit
GAAGACACCTCCATCTACCTGTCTCCGCCGC5390.10217060405763255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)