FastQCFastQC Report
Sun 21 May 2023
EGAF00007960412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548726
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC23210.422979774969657No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG22920.41769480578649454No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22390.4080360690034735No Hit
GTAGCACTCGCGTTCGAAGTCTTCGATATCAGAGTCACAGAAGG20570.37486833137121256No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG17600.32074295732296265No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA17400.3170981509897472No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16770.3056170110401184No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16720.3047058094568145No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16330.2975984371070443No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG16280.29668723552374043No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15880.28939762285730947No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA15180.2766408006910553No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14750.268804467074642No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT14600.2660708623247304No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13240.24128617925886506No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC12910.23527224880905956No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11330.2064782787766572No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10970.1999176273768693No Hit
TTCGCTCTGCAGCGCGTCGGTCTGGTCGGCCAGTGCTTCCAGTG9470.1725815798777532No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA9190.1674788510112515No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9040.1647452462613399No Hit
CGGGCTCGGTGGGTGACGATGGTGGGTGGAAACGCCACGACGAC8990.16383404467803603No Hit
GTGCTGAACATCAAAGTCCTGCAGAACGAATTTCAGGTACTCCA8410.15326410631171114No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8120.14797913712854868No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7710.14050728414545693No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6970.12702150071255963No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA6950.12665702007923807No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6800.12392341532932646No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA6700.12210101216271874No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT6270.11426467854630544No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG6170.1124422753796977No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA6160.11226003506303692No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6110.11134883347973307No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6090.1109843528464115No Hit
GAACGGCAGGCTTGCCGCAGGTTTTTTGGTACCCGGAGTCGGCG5710.10405922081330209No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5550.1011433757467297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)