FastQCFastQC Report
Wed 8 May 2024
EGAF00007960413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548726
Total Bases17 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA50860.9268742505366977No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT34940.6367476664127452No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24740.4508625434187554No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22560.4111341543867067No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22530.4105874334367244No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG22270.40584918520354424No Hit
GGCGGCGATGACAACTCTAGCAGCGGCGGTA20660.37650849422115956No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17230.314000065606514No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16800.3061637319901007No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16280.29668723552374043No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16140.2941358710904896No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG16010.29176674697389954No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT15910.2899443438072918No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14320.26096813345822867No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG12970.2363656907090242No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10790.1966373016769754No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10080.18369823919406042No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA10040.18296927792741732No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10030.18278703761075654No Hit
CCGAACGGTGGTACCCTGACTAACCTGCTGG10010.182422556977435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9370.17075917671114546No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9270.1689367735445377No Hit
GCGGACGAAGAGGAAGAGGAACTGCTGGCGC8990.16383404467803603No Hit
GAAGGCGACGAATTTTCCTTCTGCGATTCCG8900.16219388182808905No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8240.15016602092847797No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8040.1465212145952625No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7430.13540455527895526No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7370.13431111337899063No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7190.13103078767909668No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC7050.12847942324584585No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6910.12592805881259497No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT6790.12374117501266571No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6780.12355893469600493No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG6750.12301221374602261No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6330.11535812044627007No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG6320.11517588012960932No Hit
GCTGGTCACCTGATCGGTAAAGGCGCGAAAG6190.11280675601301925No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5990.10916194967980376No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5970.1087974690464822No Hit
GAAGACACCTCCATCTACCTGTCTCCGCCGC5910.10770402714651757No Hit
TCTAGCGATCTGAACGCATACGGTGTAGTTT5750.1047881820799452No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5730.10442370144662363No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5720.10424146112996285No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5720.10424146112996285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)