FastQCFastQC Report
Wed 8 May 2024
EGAF00007960414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535519
Total Bases23.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22260.4156715261269909No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21930.40950927978279017No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG21650.40428070712710473No Hit
GTAGCACTCGCGTTCGAAGTCTTCGATATCAGAGTCACAGAAGG19410.36245212588162135No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG17470.32622558676722957No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA17410.32510517834101127No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG16260.3036306835051604No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16150.30157660139042686No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT15950.29784190663636584No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15830.29560108978392924No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14980.27972863707916995No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14460.2700184307186113No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14420.2692714917677991No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT14120.2636694496367076No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12560.23453883055503166No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC12200.22781637999772184No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10760.20092657776848255No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10050.18766841139156595No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9730.1816928997850683No Hit
TTCGCTCTGCAGCGCGTCGGTCTGGTCGGCCAGTGCTTCCAGTG9160.17104901973599443No Hit
CGGGCTCGGTGGGTGACGATGGTGGGTGGAAACGCCACGACGAC8480.15835105757218698No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8110.15144187227717412No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8010.14957452490014359No Hit
GTGCTGAACATCAAAGTCCTGCAGAACGAATTTCAGGTACTCCA7780.14527962593297342No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7440.1389306448510697No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7130.13314186798227515No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA6780.12660615216266838No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA6770.12641941742496532No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6310.117829619490625No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6310.117829619490625No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT6300.11764288475292194No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG5910.11036022998250296No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5540.10345104468749008No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5530.10326430994978703No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA5480.10233063626127177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)