FastQCFastQC Report
Sun 21 May 2023
EGAF00007960432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518730
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC28940.5579010275094943No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT27900.5378520617662368No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA25010.4821390704219922No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA23660.4561139706591098No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT20170.38883426830913964No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19980.3851714764906599No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18270.35220635012434215No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA16890.3256029148111734No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14670.282806084089989No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14040.27066103753397724No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12110.23345478379889345No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11770.2269003142289823No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11760.22670753571222024No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11550.2226591868602163No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA10180.19624853006380968No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC10170.1960557515470476No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9100.17542845025350376No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8690.16752453106625798No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8330.16058450446282269No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7960.15345169934262526No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5920.11412488192315848No Hit
GCTGTAGTGACCGGTATTCAGGTACATAGACGGAATGCCCAGAG5730.11046209010467872No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5710.11007653307115454No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5580.10757041235324735No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5580.10757041235324735No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5520.10641374125267479No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT5520.10641374125267479No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5510.10622096273591271No Hit
GCGGTCACCACCGAACTGTTCACCAAACTGATCGCCGAAGAAGA5500.10602818421915061No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5300.10217261388390877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)