FastQCFastQC Report
Wed 8 May 2024
EGAF00007960450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532640
Total Bases23.4 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27840.5226794833283268No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25790.48419194953439476No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23490.44101081405827575No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA20720.3890057074196456No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT18130.3403799939921898No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG17950.3370006007810153No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT15220.285746470411535No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14750.27692249924902373No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT14430.2709146890958246No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11850.22247671973565636No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11780.22116251126464403No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10580.1986332231901472No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT10500.1971312706518474No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10390.1950660859116852No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC10030.18830729948933614No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9970.1871808350856113No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9140.17159807750075098No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8320.15620306398317813No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8140.15282367077200362No Hit
CAGCAGACCCAGATCGTACTGTTCGCCAGTTACCATGCTTTTCA8060.1513217182337038No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8050.15113397416641636No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7710.14475067587864224No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7690.1443751877440673No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7050.13235956743766897No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6910.12973115049564435No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6820.12804145389005708No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6770.1271027335536197No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6450.12109492340042055No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6170.11583808951637128No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT6090.1143361369780715No Hit
CAGCGCTTCCACCTCGCGAGAAACTGCATCAGTGAAGCCGGTGC5840.10964253529588465No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5680.10663863021928507No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5480.10288374887353559No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5450.10232051667167316No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5450.10232051667167316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)