FastQCFastQC Report
Wed 8 May 2024
EGAF00007960451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532640
Total Bases16.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44200.8298287774106338No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31090.5836963051967557No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27230.511227095223791No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25980.48775908681285673No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA24010.45077350555722434No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC20440.3837488735355963No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA20230.3798062481225593No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG18010.33812706518474017No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA16050.3013292279963953No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15800.2966356263142085No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA14160.2658455992790628No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12210.22923550615800542No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12090.22698257735055574No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11140.2091468909582457No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10760.20201261640132173No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG10520.19750675878642235No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10440.19600480624812258No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9930.1864298588164614No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9910.18605437068188646No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9210.17291228597176328No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9180.17234905376990087No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8920.16746770802042657No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT8230.15451336737759086No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8080.15169720636827877No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7690.1443751877440673No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7630.14324872334034244No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7190.1349879843796936No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7180.13480024031240614No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6800.1276659657554821No Hit
GACGACCAGGGTCAGGTCACTGCGACGCGTG6660.1250375488134575No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6420.12053169119855814No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC6290.11809101832382096No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6120.11489936917993393No Hit
CCGGAGATCAAAGGTCAGCTGCTGGGCGGTG6080.11414839291078402No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6070.11396064884349655No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5690.10682637428657255No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA5420.10175728446981075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)