FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556354
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27830.500221082260575No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT27630.4966262487552889No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24910.44773651308339657No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA22310.4010036775146759No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG19660.3533721335696337No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT19560.3515747168169906No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT16770.3014267894182481No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT15960.286867713721839No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15930.28632848869604605No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12810.23024908601358127No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12430.22341890235353748No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC11320.2034675763991991No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11190.20113093462076304No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11120.19987274289391285No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10130.18207831704274619No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC9840.17686580846008118No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9490.17057484982583032No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9230.16590156626895824No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8840.15889164093365016No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8640.15529680742836396No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8620.15493732407783534No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8030.14433256523724103No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7960.14307437351039087No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7760.13947954000510465No Hit
CAGCAGACCCAGATCGTACTGTTCGCCAGTTACCATGCTTTTCA7740.13912005665457605No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7120.12797607278818882No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT7020.1261786560355457No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6920.12438123928290262No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6730.12096614745288072No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6350.11413596379283694No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT6210.1116195803391366No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6080.10928293856070055No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5630.10119456317380661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)