FastQCFastQC Report
Sun 21 May 2023
EGAF00007960454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540209
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27050.500732124048285No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26200.4849974732001873No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24120.4464938570071954No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA22050.40817535435359276No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG19180.3550477685488394No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT18790.34782834051265343No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT15620.2891473485262186No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14870.27526383307201474No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT14720.27248712998117397No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12250.226764085751996No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11970.22158090664909322No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11220.20769739119488936No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10450.19344364866190678No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10440.1932585351225174No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10010.18529865292877387No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9960.18437308523182694No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC9210.1704895697776231No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8760.1621594605051008No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8500.1573465084809768No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8330.15419957831135728No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8180.1514228752205165No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7790.1442034471843305No Hit
CAGCAGACCCAGATCGTACTGTTCGCCAGTTACCATGCTTTTCA7700.14253742532982605No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7630.14124163055410036No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7570.14013094931776404No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7180.13291152128157804No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6570.1216195953788256No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6540.12106425476065745No Hit
CAGCGCTTCCACCTCGCGAGAAACTGCATCAGTGAAGCCGGTGC6140.11365971318508207No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT6050.1119936913305776No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6010.11125323717302008No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5730.10607005807011731No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5640.10440403621561284No Hit
CTGGGCCACGCGAGTTTCGGTCAGTGGGTTGACCGGTTTACGGG5520.10218267374294022No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5510.10199756020355084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)