FastQCFastQC Report
Wed 8 May 2024
EGAF00007960455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540209
Total Bases16.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43730.8095015077497782No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32290.5977316186883225No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26890.4977703074180549No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26820.49647451264232917No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25430.4707437306672047No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC22020.4076200137354246No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA21300.3942918388993889No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG19310.3574542445609014No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA16490.30525222645309497No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15510.28711109959293535No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA14430.26711883733888175No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12570.2326877190124563No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12320.2280598805277217No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11310.20936341304939385No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11140.20621648287977432No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11130.20603136934038493No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10790.19973750900114584No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10320.1910371726498448No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10200.18881581017717217No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG9720.1799303602864817No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9590.1775238842744197No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9140.16919377500189742No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT9000.16660218545044603No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8740.16178923342632204No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8140.15068242106295895No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7770.14383322010555175No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7320.13550311083302943No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7260.13439242959669312No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6700.1240260713908876No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6660.12328561723333006No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6480.11995357352432114No Hit
CCGGAGATCAAAGGTCAGCTGCTGGGCGGTG6400.11847266520920606No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6370.11791732459103793No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC6300.11662152981531222No Hit
GACGACCAGGGTCAGGTCACTGCGACGCGTG6140.11365971318508207No Hit
TCCACCGGTGGTAACGGCCTGGTTGGTTTCG5740.10625517160950668No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5650.10458914975500222No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5620.10403380913683409No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA5440.10070176542782516No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5430.10051665188843577No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5420.10033153834904639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)