FastQCFastQC Report
Wed 8 May 2024
EGAF00007960465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542775
Total Bases16.8 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23150.4265119064068905No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT20450.3767675371931279No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13420.2472479388328497No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG11920.21961217815853717No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11890.21905946294505088No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11500.21187416516972962No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11070.2039519137764267No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA11060.20376767537193127No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG10870.20026714568651835No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10330.19031827184376585No Hit
TCCTACACCAACGGCAAACTGACTATCACCC9250.17042052415826078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8900.16397218000092117No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8430.1553129749896366No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7790.1435217171019299No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7480.13781032656257197No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7390.13615218092211323No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7140.13154622080972778No Hit
GTCAACATCAAACACAGCGGCCGCGAAGACA6960.12822992952881027No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5910.10888489705679148No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5890.10851642024780066No Hit
ACCGCCACCGTTAAAGCATATGGCGGCGAAG5790.10667403620284648No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5770.10630555939385566No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5650.10409469853991063No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA5590.10298926811293813No Hit
GGTGCCCTGCTGAACGACAAACACTCCAACG5520.10169959928147024No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5510.10151536087697481No Hit
GGTGCACTGCTGAACGACAAACACTCCAACG5480.10096264566348855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)