FastQCFastQC Report
Wed 8 May 2024
EGAF00007960481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533067
Total Bases16.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60281.1308147005911076No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA18360.34442199573411975No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16310.30596529141740153No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC14350.2691969302170271No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13060.24499734554943373No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG12390.2324285690166527No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10470.19641058253465324No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10240.19209592790399707No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8820.16545762540168496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8750.16414446964452875No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7650.14350916488921656No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7450.13975729129734163No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7390.13863172921977912No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG7130.1337542935503417No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6980.13094038835643548No Hit
GCAGCTCACTCCCGTTCCGGCTCCGTGAGCC6800.12756370212374807No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6590.12362423485227936No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT6330.11874679918284194No Hit
GCAGATGGTACTACCGTTACGAGCGGCTCCA6190.11612048766852948No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6040.11330658247462326No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT5900.11068027096031081No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5870.11011748992152956No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5450.10223855537859218No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5360.10055021226224846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)