FastQCFastQC Report
Wed 8 May 2024
EGAF00007960487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518586
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64761.248780337301817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41680.8037239724944368No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16530.31875137392833586No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15140.29194771937537844No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13690.2639870725395595No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12850.24778918057949886No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG11960.23062712838372038No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9680.18666142163498436No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9570.184540269116405No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8590.16564272849633427No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7500.14462403535768417No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7010.1351752650476488No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6850.13208995229335155No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6610.12746198316190566No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6580.12688348702047492No Hit
GCAGATGGTACTACCGTTACGAGCGGCTCCA6500.12534083064332627No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG6360.12264118198331618No Hit
GCAGCTCACTCCCGTTCCGGCTCCGTGAGCC6290.12129135765331112No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6060.1168562205690088No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5790.11164975529613219No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5770.11126409120184502No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5520.10644329002325555No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5400.1041293054575326No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5320.10258664908038397No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5250.10123682475037891No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5220.10065832860894819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)