FastQCFastQC Report
Wed 8 May 2024
EGAF00007960499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497082
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42830.8616284637142363No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28390.5711331329639777No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21570.43393242965949275No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18400.3701602552496369No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16930.34058766964001913No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG16620.3343512740352698No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16140.32469491955049673No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14380.28928828643966187No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT13880.27922958385135654No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11720.2357759886698774No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11410.22953959306512808No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11290.22712550444393478No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10370.20861749168145297No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10240.20600222900849355No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9490.19091417512603553No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8190.16476154839644164No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8160.1641580262411433No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG7710.1551051939116685No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7260.1460523615821937No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7260.1460523615821937No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7200.14484531727159702No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7130.14343709890923428No Hit
CCGGGCGTTCGTCGTGAAATGCAGATGGTGA6840.1376030514080172No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6700.1347866146832917No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT6580.1323725260620984No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6520.13116548175150178No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6140.12352086778438968No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6110.12291734562909137No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6050.12171030131849472No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG5990.1205032570078981No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5840.11748564623140648No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5780.11627860192080985No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5600.11265746898901992No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5560.11185277278195549No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5550.11165159873018939No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5410.10883516200546389No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5340.10742694364310114No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5280.10621989933250449No Hit
CATCGTTCCCCACTGTGTCGTGATGAAGACG5170.1040069847630773No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5120.10300111450424677No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5010.10078819993481962No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4990.1003858518312874No Hit
GCTCAGGCCATCGACTATGCTGGCTACTACG4980.10018467777952128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)